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Accelerated ageing test and behaviour investigation of isoenzymes in sesame seeds Agronomy
Nery, Marcela Carlota; Rocha, Adriana de Souza; Pinho, Édila Vilela de Resende Von; Santos, Heloisa Oliveira dos; Fialho, Cintia Maria Teixeira; Nery, Fernanda Carlota.
The seeds of a sesame crop contain approximately 50% oil content, one of the main reasons for their cultivation. However, information regarding methodologies for the assessment of quality seeds of this culture are scarce. Therefore, the objective of this study was to adapt the methods of accelerated ageing tests for sesame seeds and investigate changes in the behaviour of isoenzymes. The physiological characterization of the seeds and electrophoretic analysis of the isoenzymes superoxide dismutase, esterase, catalase, alcohol dehydrogenase, malate dehydrogenase and isocitrate lyase were also performed. For the accelerated ageing test, the seeds were subjected to the traditional method, with a saturated NaCl solution, at ageing times of 0, 24, 48, 72, and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Enzymes; Oilseed; Vigour; Quality; Sesamum indicum.; Produção e tecnologia de sementes.
Ano: 2018 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/39449
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Accelerated ageing test and behaviour investigation of isoenzymes in sesame seeds Agronomy
Nery, Marcela Carlota; Rocha, Adriana de Souza; Pinho, Édila Vilela de Resende Von; Santos, Heloisa Oliveira dos; Fialho, Cintia Maria Teixeira; Nery, Fernanda Carlota.
The seeds of a sesame crop contain approximately 50% oil content, one of the main reasons for their cultivation. However, information regarding methodologies for the assessment of quality seeds of this culture are scarce. Therefore, the objective of this study was to adapt the methods of accelerated ageing tests for sesame seeds and investigate changes in the behaviour of isoenzymes. The physiological characterization of the seeds and electrophoretic analysis of the isoenzymes superoxide dismutase, esterase, catalase, alcohol dehydrogenase, malate dehydrogenase and isocitrate lyase were also performed. For the accelerated ageing test, the seeds were subjected to the traditional method, with a saturated NaCl solution, at ageing times of 0, 24, 48, 72, and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 5.01.00.00-9 5.01.03.00-8 enzymes; Oilseed; Vigour; Quality; Sesamum indicum. Produção e tecnologia de sementes.
Ano: 2018 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/39449
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‘Desiccation tolerance of Rhamnidium elaeocarpum Reissek (Rhamnaceae) seeds Agronomy
Silva, Lílian Abadia da; Sales, Juliana de Fátima; Oliveira, João Almir; Pinho, Édila Vilela de Resende Von; Santos, Heloisa Oliveira dos; Soares, Marcos Antônio.
This study aimed to investigate the desiccation tolerance of Rhamnidium elaeocarpum Reissek seeds through physiological and biochemical alterations. Fruit were collected from the municipality of Jaupaci, Goias State, Brazil, when the water content of their seeds had a 37% wet basis (w.b.) and the seeds were subsequently kept in a drying oven with air circulation at a temperature of 35ºC until a water content was reached of 20 to 12% (w.b.). The following parameters were evaluated: germination, germination speed index, emergence, emergence speed index, electrical conductivity and respiration rate. Furthermore, the electrophoretic profile of the isoenzymes: catalase, peroxidase and esterase, the enzymatic activities of endo-β-mannanase and α-amylase and the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 5.01.03.00-8 drying; Germination; Isoenzymes; Respiration rate; Heat-resistant proteins Produção e beneficiamento de sementes.
Ano: 2015 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/19425
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Physiological analysis and gene expression analysis of ZmDBP3, ZmALDH9, ZmAN13, and ZmDREB2A in maize lines Agronomy
Marques, Thaís Lima; Pinho, Renzo Garcia Von; Pinho, Édila Vilela de Resende Von; Paniago, Bruno da Costa; Freitas, Natália Chagas; Santos, Heloisa Oliveira dos.
The objective of this study was to evaluate gene expression related to water deficit tolerance in maize lines. For this, lines previously classified as tolerant (91-T and 32-T) and non-tolerant (24-NT and 57-NT) to water deficit were used. The seeds of the four lines were evaluated for emergence and emergence speed index, and the seedlings were evaluated for root and shoot length under two conditions of water availability (70 and 10% substrate water retention capacity). In transcript analysis, the expression of several genes associated with water deficit tolerance, ZmDBP3, ZmALDH9, ZmAN13, and ZmDREB2A, was evaluated by qRT-PCR for the 91-T and 57-NT lines. It can be concluded that soil water deficiency did not reduce root development. However, the shoot...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Abiotic stress; Zea mays; QRT-PCR.; Water deficit; Genética Vegetal.
Ano: 2019 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/43479
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